Agilent Poroshell 120 EC-C18 column 3.0x50mm 2.7u
Item No. 699975-302 can replace 699975-902 (4.6mm)
699975-902
Transfer traditional methods to a fast method using Agilent Poroshell 120 columns for ginseng analysis
In this paper, the traditional method of ginseng analysis using a 4.6 × 150 mm, 5 μm column was transferred to an Agilent Poroshell 120 EC-C18, 3.0 mm × 100 mm, 2.7 μm column. At the same line speed, the required gradient time is reduced from more than 100 minutes to approximately 70 minutes, and if the flow rate is increased, the analysis time can be further reduced to less than 25 minutes. In this example, the pressure exceeds 400 bar at high flow rates, so a 600 bar instrument is required.
If an Agilent Poroshell 120 EC-C18 or Poroshell 120 SB-C18 3.0 mm × 50 mm column is used, the gradient time can be further reduced. The shortest time is 11 minutes and the resolution of the key compounds (ginsenoside Rg1 and Re) is 1.57.