COSMOSIL SUGAR-D Sugar Analysis Column 05397-51 Column High-water mobile phase
- In the past, monosaccharides and oligosaccharides were analyzed by liquid chromatography using an aminopropyl-bonded stationary phase. The disadvantages of this column are peak tailing, short column life, and small adsorption to certain sugars. However, COSMOSIL® Sugar-D solves these problems with better separation and sharp peaks, which increases column life. With 100% water as the mobile phase, COSMOSIL ® Sugar-D has a low retention time. Compared to traditional C18 columns, COSMOSIL® Sugar-D is more conducive to the separation of highly hydrophilic compounds.
The aminopropyl column only partially adsorbs certain sugars such as aldose or galactose, and the sample disappears or peaks are tailed. COSMOSIL® Sugar-D analyzes these sugars to obtain a sharp peak shape.
Chromatogram
COSMOSIL® Sugar-D Aminopropyl type
Separation conditions:
Column size: 4.6mmI.D. × 150mm
Mobile phase: acetonitrile: water = 70:30
Flow rate: 1ml/min
Temperature: 30 °C
Detector: RI
Sample:
1. Arabinose (10μg)
2. Galactose (10μg)
3. Maltitol (10μg)